In Frontiers in cellular neuroscience ; h5-index 74.0
Quantitative and mechanistic understanding of learning and long-term memory at the level of single neurons in living brains require highly demanding techniques. A specific need is to precisely label one cell whose firing output property is pinpointed amidst a functionally characterized large population of neurons through the learning process and then investigate the distribution and properties of dendritic inputs. Here, we disseminate an integrated method of daily two-photon neuronal population Ca2+ imaging through an auditory associative learning course, followed by targeted single-cell loose-patch recording and electroporation of plasmid for enhanced chronic Ca2+ imaging of dendritic spines in the targeted cell. Our method provides a unique solution to the demand, opening a solid path toward the hard-cores of how learning and long-term memory are physiologically carried out at the level of single neurons and synapses.
Huang Junjie, Liang Susu, Li Longhui, Li Xingyi, Liao Xiang, Hu Qianshuo, Zhang Chunqing, Jia Hongbo, Chen Xiaowei, Wang Meng, Li Ruijie
2023
auditory cortex, behaving mouse, daily two-photon Ca2+ imaging, dendritic spines, loose-patch recording
