In The Journal of physiology ; h5-index 67.0
Because of the biophysical relation between muscle fibre diameter and the propagation velocity of action potentials along the muscle fibres, motor unit conduction velocity could be a non-invasive index of muscle fibre size in humans. However, the relation between motor unit conduction velocity and fibre size has been only assessed indirectly in animal models and in human patients with invasive intramuscular EMG recordings, or it has been mathematically derived from computer simulations. By combining advanced non-invasive techniques to record motor unit activity in vivo, i.e., high-density surface EMG, with the gold standard technique for muscle tissue sampling, i.e., muscle biopsy, here we investigated the relation between the conduction velocity of populations of motor units identified from the biceps brachii muscle, and muscle fibre diameter. We demonstrate the possibility to predict muscle fibre diameter (R2 = 0.66) and cross-sectional area (R2 = 0.65) from conduction velocity estimates with low systematic bias (∼2% and ∼4% respectively) and a relatively low margin of individual error (∼8% and ∼16%, respectively). The proposed neuromuscular interface opens new perspectives in the use of high-density EMG as a non-invasive tool to estimate muscle fibre size without the need of surgical biopsy sampling. The non-invasive nature of high-density surface EMG for the assessment of muscle fibre size may be useful in studies monitoring child development, aging, space and exercise physiology, although the applicability and validity of the proposed methodology needs to be more directly assessed in these specific populations by future studies. KEY POINTS: Because of the biophysical relation between muscle fibre size and the propagation velocity of action potentials along the sarcolemma, motor unit conduction velocity could represent a potential non-invasive candidate to estimate muscle fibre size in vivo. This relation has been previously assessed in animal models and humans with invasive techniques, or it has been mathematically-derived from simulations. By combining high-density surface EMG with muscle biopsy, here we explored the relation between the conduction velocity of populations of motor units and muscle fibre size in healthy individuals. Our results confirmed that motor unit conduction velocity can be considered as a novel biomarker of fibre size, which can be adopted to predict muscle fibre diameter and cross-sectional area with low systematic bias and margin of individual error. The proposed neuromuscular interface opens new perspectives in the use of high-density EMG as a non-invasive tool to estimate muscle fibre size without the need of surgical biopsy sampling Abstract figure legend In this study, we investigated the relation between the conduction velocity of populations of motor units identified from biceps brachii muscle and muscle fibre size. We adopted high-density surface EMG to decode the activity of voluntarily activated motor units and estimated their conduction velocity. Similarly, we adopted muscle biopsy to measure muscle fibre size. We revealed the possibility to accurately transform motor unit conduction velocity values into estimated measures of muscle fibre size, which in turn showed a good degree of association with the muscle fibre size measured directly by muscle biopsy. Furthermore, we demonstrated that the proposed neuromuscular interface allows to predict the mean measured fibre diameter and cross-sectional area from an EMG-derived parameter with a relatively low bias and error, thus opening new perspectives in the use of high-density EMG as a non-invasive tool to estimate muscle fibre size without the need of surgical biopsy sampling This article is protected by copyright. All rights reserved.
Casolo Andrea, Maeo Sumiaki, Balshaw Thomas G, Lanza Marcel B, Martin Neil R W, Nuccio Stefano, Moro Tatiana, Paoli Antonio, Felici Francesco, Maffulli Nicola, Eskofier Bjoern, Kinfe Thomas M, Folland Jonathan P, Farina Dario, Vecchio Alessandro Del
2023-Mar-16
Motor unit, conduction velocity, high-density surface electromyography, muscle fibre size
