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A Cre-LoxP-based approach for combinatorial chromosome rearrangements in human HAP1 cells.

In Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology

Alterations of human karyotype caused by chromosomal rearrangements are often associated with considerable phenotypic effects. Studying molecular mechanisms underlying these effects requires an efficient and scalable experimental model. Here, we propose a Cre-LoxP-based approach for the generation of combinatorial diversity of chromosomal rearrangements. We demonstrate that using the developed system, both intra- and inter-chromosomal rearrangements can be induced in the human haploid HAP1 cells, although the latter is significantly less effective. The obtained genetically modified HAP1 cell line can be used to dissect genomic effects associated with intra-chromosomal structural variations.

Khabarova Anna, Koksharova Galina, Salnikov Pavel, Belokopytova Polina, Mungalov Roman, Pristyazhnuk Inna, Nurislamov Artem, Gridina Maria, Fishman Veniamin

2023-Feb-26

Cell line models, Chromosome rearrangement, Cre/LoxP system, Genome editing