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In Plant communications

With the development of high throughout biology techniques and artificial intelligence, it becomes increasingly feasible to design and construct artificial biological parts, modules, circuits and even biological systems. To overcome the limitations of native promoters in controlling gene expression, artificial promoter design aims to synthesize short, inducible and conditionally controlled promoters to coordinate multiple genes' expressions in diverse plant metabolic and signalling pathways. Synthetic promoters are capable of driving gene expression accurately with smart responses and have versatile usages, highlighting their potentials to enhance desirable traits in crops, thereby improving crop yield, nutritional quality and food security. This review first illustrates the importance of synthetic promoters, then introduces promoter architecture and thoroughly summarizes the advances of synthetic promoter construction. The restrictions of developing synthetic promoters and their future applications in synthetic plant biology and crop improvement are also discussed.

Yasmeen Erum, Wang Jin, Riaz Muhammad, Zhang Lida, Zuo Kaijing

2023-Feb-08

Plants, cis-element, gene expression, synthetic promoter