In Biosensors & bioelectronics
Cholera is a highly contagious and lethal waterborne disease induced by an infection with Vibrio cholerae (V. cholerae) secreting cholera toxin (CTx). Cholera toxin subunit B (CTxB) from the CTx specifically binds with monosialo-tetra-hexosyl-ganglioside (GM1) found on the exterior cell membrane of an enterocyte. Bioinspired by the pathological process of CTx, we developed an electrochemical biosensor with GM1-expressing Caco-2 cell membrane (CCM) on the electrode surface. Briefly, the electrode surface was functionalized with CCM using the vesicle fusion method. We determined the CTxB detection performances of Caco-2 cell membrane-coated biosensor (CCB) using electrochemical impedance spectroscopy (EIS). the CCB had an excellent limit of detection of ∼11.46 nM and a detection range spanning 100 ng/mL - 1 mg/mL. In addition, the CCB showed high selectivity against various interfering molecules, including abundant constituents of intestinal fluid and various bacterial toxins. The long-term stability of the CCBs was also verified for 3 weeks using EIS. Overall, the CCB has excellent potential for practical use such as point-of-care and cost-effective testing for CTxB detection in developing countries.
Kim Yonghwan, Lee Dongtak, Seo Youngjun, Jung Hyo Gi, Jang Jae Won, Park Dongsung, Kim Insu, Kim Jaeheung, Lee Gyudo, Hwang Kyo Seon, Kim Seung-Hyun, Lee Sang Won, Lee Jeong Hoon, Yoon Dae Sung
2023-Jan-28
Bioinspired, Biosensor, Caco-2 cell membrane, Cholera, Cholera toxin subunit B, Electrochemical impedance spectroscopy
