In Nature communications ; h5-index 260.0
Aberrant pro-survival signaling is a hallmark of cancer cells, but the response to chemotherapy is poorly understood. In this study, we investigate the initial signaling response to standard induction chemotherapy in a cohort of 32 acute myeloid leukemia (AML) patients, using 36-dimensional mass cytometry. Through supervised and unsupervised machine learning approaches, we find that reduction of extracellular-signal-regulated kinase (ERK) 1/2 and p38 mitogen-activated protein kinase (MAPK) phosphorylation in the myeloid cell compartment 24 h post-chemotherapy is a significant predictor of patient 5-year overall survival in this cohort. Validation by RNA sequencing shows induction of MAPK target gene expression in patients with high phospho-ERK1/2 24 h post-chemotherapy, while proteomics confirm an increase of the p38 prime target MAPK activated protein kinase 2 (MAPKAPK2). In this study, we demonstrate that mass cytometry can be a valuable tool for early response evaluation in AML and elucidate the potential of functional signaling analyses in precision oncology diagnostics.
Tislevoll Benedicte Sjo, Hellesøy Monica, Fagerholt Oda Helen Eck, Gullaksen Stein-Erik, Srivastava Aashish, Birkeland Even, Kleftogiannis Dimitrios, Ayuda-Durán Pilar, Piechaczyk Laure, Tadele Dagim Shiferaw, Skavland Jørn, Panagiotis Baliakas, Hovland Randi, Andresen Vibeke, Seternes Ole Morten, Tvedt Tor Henrik Anderson, Aghaeepour Nima, Gavasso Sonia, Porkka Kimmo, Jonassen Inge, Fløisand Yngvar, Enserink Jorrit, Blaser Nello, Gjertsen Bjørn Tore
2023-Jan-07
