In Metabolic engineering
Chinese hamster ovary (CHO) cells are extensively used for the production of glycoprotein therapeutics proteins, for which N-linked glycans are a critical quality attribute due to their influence on activity and immunogenicity. Manipulation of protein glycosylation is commonly achieved through cell or process engineering, which are often guided by mathematical models. However, each study considers a unique glycosylation reaction network that is tailored around the cell line and product at hand. Herein, we use 200 glycan datasets for both recombinantly produced and native proteins from different CHO cell lines to reconstruct a comprehensive reaction network, CHOGlycoNET, based on the individual minimal reaction networks describing each dataset. CHOGlycoNET is used to investigate the distribution of mannosidase and glycosyltransferase enzymes in the Golgi apparatus and identify key network reactions using machine learning and dimensionality reduction techniques. CHOGlycoNET can be used for accelerating glycomodel development and predicting the effect of glycoengineering strategies. Finally, CHOGlycoNET is wrapped in a SBML file to be used as a standalone model or in combination with CHO cell genome scale models.
Kotidis Pavlos, Donini Roberto, Arnsdorf Johnny, Hansen Anders Holmgaard, Voldborg Bjørn Gunnar Rude, Chiang Austin W T, Haslam Stuart, Betenbaugh Michael, Jimenez Del Val Ioscani, Lewis Nathan E, Krambeck Frederick, Kontoravdi Cleo
2023-Jan-04
Chinese hamster ovary cells, Glycoengineering, Protein glycosylation, Systems glycobiology
