In Journal of separation science
Hydrophobicity is an important physicochemical property of peptides and proteins. It is responsible for their conformational changes, stability, as well as various chemical intramolecular and intermolecular interactions. Enormous efforts have been invested to study the extent of hydrophobicity and how it could influence various biological processes, in addition to its crucial role in the separation and purification endeavour as well. Here, we have reviewed various studies that were carried out to determine the hydrophobicity starting from: (i) simple amino acids solubility behaviour; (ii) experimental approach that was undertaken in the reversed-phase liquid chromatography mode; and ending with (iii) some examples of more advanced computational and machine learning models. This article is protected by copyright. All rights reserved.
Al Musaimi Othman, Valenzo Oscar M Mercado, Williams Daryl R
2022-Nov-09
hydrophobicity, machine learning, peptides, retention behaviour
