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In Journal of neurochemistry

Over the past decade, novel optical imaging tools have been developed for imaging neuronal activities along with the evolution of fluorescence indicators with brighter expression and higher sensitivity. Miniature microscopes, as revolutionary approaches, enable the imaging of large populations of neuron ensembles in freely behaving rodents and mammals, which allow exploring the neural basis of behaviors. Recent progress on two-photon miniature microscopes and mesoscale single-photon miniature microscopes further expands those affordable methods to navigate neural activities during naturalistic behaviors. In this review article, two-photon miniature microscopy techniques are summarized historically from the first documented attempt to the latest ones, and comparisons are made. The driving force behind and their potential for neuroscientific inquiries are also discussed. Current progress in terms of the mesoscale, i.e., the large field-of-view miniature microscopy technique, is addressed as well. Then, pipelines for registering single cells from the data of two-photon and large field-of-view miniature microscopes are discussed. Finally, we present the potential evolution of the techniques.

Guo Changliang, Wang Aimin, Cheng Heping, Chen Liangyi


Large field-of-view imaging, Miniature fluorescence microscopy, Neural imaging, Neuroscience, Two-photon microscopy