In Microscopy research and technique
The evaluation of oocyte viability in the laboratory is limited to the morphological assessment by naked eyes, but the realization that most normal-appearing oocytes may conceal abnormalities prompts the search for automated approaches that can detect the abnormalities imperceptible to naked eyes. In this study, we developed an image processing pipeline applicable to bright-field microscope images to quantify the causal relationship between the quantitative imaging features and the developmental potential of oocytes. We acquired 19 imaging features of approximately 700 oocytes and determined two imaging subtypes, namely viable and nonviable subtypes that correlated closely with a viability fluorescence indicator and cleavage rates. The causal relationship between these imaging features and oocyte viability was derived from a viability-oriented Bayesian network that was developed based on the Bayesian information criterion and Tabu search. Our experimental results revealed that entropy with mean Gray Level Co-Occurrence Matrix energy describing the uniformity and texture roughness of cytoplasm were salient features for the automated selection of promising oocytes that exhibited excellent developmental potential.
Chen Yizhe, Liu Yaowei, Zuo Xiaoying, Zhao Qili, Sun Mingzhu, Cui Maosheng, Zhao Xin, Du Yue
2022-Oct-24
Bayesian network, cytoplasmic texture, entropy, oocyte viability, quantitative imaging features
