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Pipeline for fluorescent imaging and volumetric analysis of neurons in cleared mouse spinal cords.

In STAR protocols

Precisely measuring the number and somatic volume of neurons in the central nervous system at single-cell resolution is technically challenging. Here, we combine multiple techniques to address this challenge in optically cleared mouse spinal cords. We describe in vivo neuron labeling approaches, tissue-clearing technology, light sheet fluorescence microscopy, and machine learning-guided imaging analysis. This combination provides a precise determination of the cell number and somatic volume of any neuron population in the spinal cords.

Yu Hao, Li Qiang, Sandoval Alfredo, Gibbs Holly C, English Amber, Dunn Tiffany, Moth John, Elahi Hajira, Chen Bo

2022-Oct-11

Cell Biology, Microscopy, Molecular/Chemical Probes, Neuroscience