In The American journal of pathology ; h5-index 54.0
Differentiating between indolent and aggressive prostate cancers (CaP) is important to decrease over-treatment and increase survival for men with aggressive disease. Nucleolar prominence is a histologic hallmark of CaP; however, the expression, localization, and functional significance of specific nucleolar proteins has not been thoroughly investigated. Nucleolar protein BOP1 has been associated with multiple cancers, but it has not been previously implicated in CaP. Meta-analysis of publicly available data showed increased BOP1 expression in metastatic CaP, recurrent CaP, and was inversely associated with overall survival. Multiplexed immunohistochemistry was used to analyze the expression and localization of BOP1 and nucleolar marker NOP56 in human tissue samples from various stages of CaP progression. Here, increased BOP1 expression was observed at later stages of CaP progression, coinciding with a localization change from nuclear to cytoplasmic. In patient samples, cytoplasmic BOP1 was also inversely associated with overall survival. In models of prostate cancer progression, BOP1 expression showed similar expression and localization to the human patient samples. Functional significance of BOP1 in metastatic CaP was assessed by genetic knockdown, where knockdown of BOP1 resulted in decreased proliferation and motility compared to control. Taken together, these data suggest a prognostic significance of BOP1 expression and localization in CaP progression and provide a foundation for further investigation into the functional role of nucleolar proteins in advanced CaP.
Vellky Jordan E, Ricke Emily A, Huang Wei, Ricke William A
biomarker, machine-learning, multispectral imaging, nucleolus, recurrence