Receive a weekly summary and discussion of the top papers of the week by leading researchers in the field.

In Biomedical optics express

Obtaining fine structures of neurons is necessary for understanding brain function. Simple and effective methods for large-scale 3D imaging at optical resolution are still lacking. Here, we proposed a deep-learning-based fluorescence micro-optical sectioning tomography (DL-fMOST) method for high-throughput, high-resolution whole-brain imaging. We utilized a wide-field microscope for imaging, a U-net convolutional neural network for real-time optical sectioning, and histological sectioning for exceeding the imaging depth limit. A 3D dataset of a mouse brain with a voxel size of 0.32 × 0.32 × 2 µm was acquired in 1.5 days. We demonstrated the robustness of DL-fMOST for mouse brains with labeling of different types of neurons.

Ning Kefu, Zhang Xiaoyu, Gao Xuefei, Jiang Tao, Wang He, Chen Siqi, Li Anan, Yuan Jing