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In Journal of microbiological methods ; h5-index 0.0

With qPCR reaching thousands of reactions per run, assay validation needs automation. We applied support vector machine to qPCR analysis and we could identify reactions with 100% accuracy, dispensing them from further validation. We achieved a greatly reduced workload that could improve high-throughput qPCR analysis.

Marongiu Luigi, Shain Eric, Shain Kevin, Allgayer Heike


Assay accuracy, High throughput, SVM, Validation, maxRatio, qPCR